Year: 2015 | Month: September | Volume 8 | Issue 3

Molecular characterization of ridge gourd (Luffa acutangula L.) and sponge gourd (Luffa cylindrica l.) genotypes through PCR based molecular markers



The present study was carried out for the comparison of RAPD and ISSR markers for polymorphism pa ern and molecular diversity analysis among 17 ridge gourd (Luff a acutangula L.) and sponge gourd (Luff a cylindrica L.) genotypes using 20 RAPD and 12 ISSR markers. Twenty RAPD primers generated total of 94 bands of which 81 bands were polymorphic showing 86.17% polymorphism. The average bands per primer were found 4.05. The polymorphic information content (PIC) was recorded from 0.4828 to 0.8842 for RAPD. Jaccard’s similarity coeffi cient ranged from 30.8% to 78.6% for RAPD. However, out of 30 ISSR primers screened, twelve ISSR primers produced 79 bands of which 66 bands were polymorphic and 83.54% polymorphism with an average of 7.16 bands per primer. The PIC ranged between 0.6548 and 0.8939 for ISSR. Jaccard’s similarity coeffi cient ranged from 22.7 % to 81.2% for ISSR. This study showed that RAPD and ISSR markers produced specifi c DNA fragments for identifi cation of ridge gourd and sponge gourd genotypes.

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International Journal of Agriculture Environment & Biotechnology(IJAEB)| In Association with AAEB

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