Year: 2013 | Month: November | Volume 6 | Issue 4s
Cellulase from Cashew Shell Cake using Aspergillus niger Production, Purification and Potential Applications
Abstract: <div>The Cellulose content in the Cashew Shell Cake was utilized for the production of enzyme Cellulase CEPC-C11; induced by Aspergillus niger (MTCC- 1344) using the Solid-State Fermentation (SSF). Optimised conditions by SSF for Cellulase production were at 30ºC, pH 7.0 and 216 hours of incubation. The enzyme activity of Cellulase was found through C1 and Cx Cellulase Combined Assay. Partially Purified Cellulase was concentrated and powdered by acetone precipitation method. Purified Cellulase showed a maximum specific enzyme activity of 15.322 U/mg of protein at 50ºC, pH 5.0 and on 30 minutes of incubation. Thus cellulase enzyme yield of 20% /Kg of substrate showing Km and Vmax of 1.786 and 19.45 U/mg respectively was obtained. This enzyme which is capable of hydrolyzing native cellulose; that has functional temperature ranging from 25ºC to 70ºC and pH ranging from 3.5 to 8.0 respectively; could find various industrial applications</div>
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