Year: 2013 | Month: November | Volume 6 | Issue 4s
A Novel Approach for Enhancement of Lovastatin Production using Aspergillus Species
Abstract: <div>Submerged cultures (SmF) of filamentous fungi (Aspergillus terreus, Aspergillus flavus) are widely used to produce commercially important metabolite lovastatin. Limited investigations have been made on optimization of the different nutrients and process parameters using standard optimization methods. No previous work has used statistical analysis in documenting the interactions between nutritional and process parameters in lovastatin production. With this lacunae, Plackett-Burman experimental design</div><div>was used for the first time to screen and investigate the effects of the nine factors —i.e. Source (lactose, glycerol and honey) and concentrations of carbon (40–80 g/L), source (mycological peptone and yeast extract) and concentrations of Nitrogen (5–25 g/L); pH of the fermentation process (6.0-7.6); Temperature of the fermentation process (24 <sup>o</sup>C –32 <sup>o</sup>C) ; Agitation (120 – 200 rpm) and Fermentation time (5–13 days) —on the concentrations of lovastatin produced in batch cultures (SmF) of Aspergillus terreus</div><div>(newly discovered strains (nhceup) MTCC-11045, Aspergillus terreus (NHCEUPBT) MTCC-11395, Aspergillus flavus (NHCEUPBTE) MTCC-11396 and Aspergillus terreus MTCC-1782. Lovastatin in the sample was confirmed and estimated by UV Spectrophotometry, HPLC and FTIR analysis; Plackett-Burman design identified the “source and concentrations of C, N, pH and incubation period” were the principal factor influencing the production of lovastatin.</div>
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